| Title | Pseudomonas putida에서 분리한 R-factor pKU 10의 유전자 지도 | ||
| Author | 임영복 · 민경미 · 이영록 | ||
| Address | 고려대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(3),167-172, 1988 | ||
| DOI | |||
| Key Words | vector in Pseufomonasm genetic map | ||
| Abstract | A genetic map of the IncP-1 group plasmid pKU10 has been prepared through the construction of recombinant plasmids containing various fragments of pKU10. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to ampicillin, tetracyclin, and chloramphenicol. The region involved in conferring resistance to ampicillin was located around two PstI sites that are 1.0Kb apart. The tetracyclin resistance gene was mapped on the region of HindIII E fragment and a part of HindIII D fragment, and the determinant for chloramphenicol resistance gene was localized on HindIII D fragment. | ||
| Download PDF | Kor_260304_167-172p.pdf | ||