| Title | pEA 9::Tn5-Mob에 의한 nif-plasmid pEA 9의 transfer 성질 | ||
| Author | 민병환 · 이호자 | ||
| Address | 경희대학교 문리과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(3),181-187, 1988 | ||
| DOI | |||
| Key Words | pEA9, RP4-mobilising system, cinjugation frequency, pBM 339 | ||
| Abstract | Using a Tn5-Mob system, pEA9 was characterized as a self-transmissible plasmid carrying a kanamycin resistance marker. The self-transfer frequencies of pEA9 varied greatly depending on pH values. The transfer frequency was about 4×10^-5 at pH 5, that was 10 times higher than one at pH 6.5. With a helper plasmid, transfer frequencies were increased about 10^4 times than the frequencies obtained without it. | ||
| Download PDF | Kor_260306_181-187p.pdf | ||