| Title | 유당 자화 효모간의 원형질체 융합 | ||
| Author | 최원기 · 전순배 * · 이용규 ** · 배석 * · 이진종 * · 이향 * | ||
| Address | 전남대학교 화학과; * 생물학과; ** 낙농학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(3),188-196, 1988 | ||
| DOI | |||
| Key Words | Saccharomyces cerevisiae, lactose assimilation, protoplast fusion, alcohol production | ||
| Abstract | Intergeneric or intraspecific protoplast fusion between Saccharomyces cerevisiae X-2180-1A, Candida pseudotropicalis ATCC 8619 and CBS 607 was attempted to produce ethanol from lactose containing materials. Teh intergeneric fusion frequency between Saccharomyces cerevisiae X-2180-1A (ade rho) and Candida pseudotropicalis CBS 607 (his met) was 1.0×10^-5. These values exhibited approximately 2-3.5 fold increase when compared with fusion frequency obtained without the treatment of bovine serum albumin, myoinositol and ergosterol, suggesting that these compounds may improve intergeneric of intraspecific protoplast fusion. Nuclear fusion appears to occur in fusants between intergenera(S. cerevisiae+C. pseudotropicalis) and intraspecies (C. pseudotropicalis strains) as strongly suggested by DNA content, nuclear staining, comparison of survival rate to UV light and isolation of recombinants after mitotic segragation. It was also found that alcohol production from intraspecific hybrids was somewhat increased when compared with that from their parents. | ||
| Download PDF | Kor_260307_188-196p.pdf | ||