Title 5-Fluorocytosine에 기질특이성을 가지는 Cytosine Deaminase의 특성
Author 이인 · 박찬영 *
Address 인제대학교 의학과; * 부산대학교 고분자공학과
Bibliography Korean Journal of Microbiology, 26(3),207-214, 1988
DOI
Key Words Cytosine Deaminase, Deamination of 5-Fluorocytosine
Abstract A cytosine deaminase from the cell-free extract of an isolate was examined after ethyl alcohol reactionation. The enzyme catalyzed the conversion of 5-fluorocytosine to 5-fluorouracil by the possession of specificity to the substrate. The optimum temperature and storage time on the stability of the enzyme were at below 50℃ and near 2 days in tris-HCl buffer. The maximum activity was also presented ar 9.0 in pH and 45℃ in temperature. The pHs and temperatures for the enzyme activity ranged from 8.5-9.5 and from 40-50℃, respectively. The presence of Ag^+, Hg^2+, Zn^2+ in the reaction mixture resulted in the marked inhibition in the activity, but 1mM of Fe^3+, K^+, or Na^+ increased the enzyme activity. The enzyme preparation was not affected by inhibitors used except N-ethylmaleimide of 1 and 10mM, and considerably activated by 1mM of pyrophosphate and 10mM of phosphate.
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