| Title | 5-Fluorocytosine에 기질특이성을 가지는 Cytosine Deaminase의 특성 | ||
| Author | 이인 · 박찬영 * | ||
| Address | 인제대학교 의학과; * 부산대학교 고분자공학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(3),207-214, 1988 | ||
| DOI | |||
| Key Words | Cytosine Deaminase, Deamination of 5-Fluorocytosine | ||
| Abstract | A cytosine deaminase from the cell-free extract of an isolate was examined after ethyl alcohol reactionation. The enzyme catalyzed the conversion of 5-fluorocytosine to 5-fluorouracil by the possession of specificity to the substrate. The optimum temperature and storage time on the stability of the enzyme were at below 50℃ and near 2 days in tris-HCl buffer. The maximum activity was also presented ar 9.0 in pH and 45℃ in temperature. The pHs and temperatures for the enzyme activity ranged from 8.5-9.5 and from 40-50℃, respectively. The presence of Ag^+, Hg^2+, Zn^2+ in the reaction mixture resulted in the marked inhibition in the activity, but 1mM of Fe^3+, K^+, or Na^+ increased the enzyme activity. The enzyme preparation was not affected by inhibitors used except N-ethylmaleimide of 1 and 10mM, and considerably activated by 1mM of pyrophosphate and 10mM of phosphate. | ||
| Download PDF | Kor_260309_207-214p.pdf | ||