| Title | 색소기질을 이용한 bacillus subtilis의 β-glucanase 정제 | ||
| Author | 이성택 · 양진오 · 정안식 | ||
| Address | 한국과학기술대학 자연과학부 | ||
| Bibliography | Korean Journal of Microbiology, 26(3),223-229, 1988 | ||
| DOI | |||
| Key Words | bacillus subtilis K-4-3, β-glucanase, chromogenic substrate | ||
| Abstract | Bacillus subtilis K-4-3, which produces considerable amount of β-glucanase was selected among extracellular β-glucanase-producing bacteria isolated from soil. β-glucanase was purified by ammonium sulfate fractionation, Sephadex G-100 gel filtration and DEAE-sephacel ion exchange chromatography. The purified enzyme revealed a single band by polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis. Its molecular weight was estimated to be 17000 dalton by SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature of the purified β-glucanase were 7.0 and 50℃, respectively. The enzyme was strongly inhibited by 1.0mM of Fe^3+ , and activated by 1.0mm of Li^4+. The absence of glucose after thin layer chromatography of reaction products revealed that the purified enzyme contains no cellobiase or laminarinbiase activity. The liberation of di, tri-and tetra-saccharide as reaction products can be explained by endoaction of the enzyme. | ||
| Download PDF | Kor_260311_223-229p.pdf | ||