| Title | 리보스 결합단백질의 대량생산을 위한 야생형 수송결합변이, 복귀변이 유전자의 클로닝과 이들 단백질의 순수정제 | ||
| Author | 박순희1 · Linda L. Randall2 | ||
| Address | 1워싱턴 주립대학교 미생물학과; 2워싱턴 주립대학교 생화학/생물리학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(4),291-297, 1988 | ||
| DOI | |||
| Key Words | E. coli, Protein Export, Ribose-binding proteins, Purification | ||
| Abstract | Three alleles of rbsB gene, rbsB, rbsB103, and rbsB106 from the wild type, the mutant and the revertant strain, respectively, were cloned for overproduction of proteins under the control of lambda P_L promoter. Five different species of precursor and mature ribose-binding proteins were purified to homogeneity using DEAE-Sephadex column chromatography, osmotic shock pocedure, CM-Sephadex column chromatography, and Chromatofocusing column chromaography. pI of the precursor proteins and mature proteins were determined and found to be pH 8.0 and 7.5, respectively. The purified proteins were subjected to amino acid sequencing. The results confirmed the amino acid changes deduced from the DNA sequencing. | ||
| Download PDF | Kor_260402_291-297p.pdf | ||