| Title | Pleurotus ostreatus에서 분리한 아스콜빈산 산화효소의 특성 | ||
| Author | 황윤엽 · 김연란 · 강사욱 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(4),324-331, 1988 | ||
| DOI | |||
| Key Words | Pleurotus ostreatus, ascorbate oxidizing enzyme | ||
| Abstract | Ascorbate oxidizing enzyme from the crude extract of Pleurotus ostreatus was purified by ammonium sulfate precipitation, preparative polyacrylamide gel electrophoresis, DEAE Sepharose CL-6B ion exchange chromatography and Sephadex G-150 gel filtration chromatography. The molecular weight of the enzyme estimated by Sephadex G-150 gel filtration chromatography was 140,000 and that of its subunit by SDS-polyacrylamide gel electrophoresis 66,000. The optimum pH for the maximum activity of the enzyme was 5.2 and the isoelectric point of the enzyme was 6.0 Km values for L-ascorbic acid and D-isoascorbic acid were both 2.2uM, which indicates that the enzyme has the same affinity towards both substrates. | ||
| Download PDF | Kor_260407_324-331p.pdf | ||