| Title | 세포외 cytosine deaminase의 효소학적 성질 | ||
| Author | 유대식 · 김대현 · 박정문 · 송형익 * · 정기택 * | ||
| Address | 계명대학교 자연과학대학 미생물학과; * 경북대학교 농과대학 식품공학과 | ||
| Bibliography | Korean Journal of Microbiology, 26(4),368-374, 1988 | ||
| DOI | |||
| Key Words | Bacillus polymyxa YL38-3, extracellular cytosine deaminase | ||
| Abstract | Enzymological proprties of an extracellular cytosine deaminase from Bacellus polymyxa YL 38-3 were investigated. The extracellular enzyme was very stable, and optimum pH and temperature for the enzyme activity were found to be near pH 6.0 in 0.2M potassium phosphate buffer and at 30℃, respectively. 5-Fluorocytosine was converted to 5-fluorouracil by the enzyme, but 5-methylcytosine was not to thymine by it. The enzyme activity was completely inhibited by some heavy metal ion such as 1mM of Cd^2- and Hg^ 2+, and by 1mM of p-chloromercuribenzoate, respectively. The enzyme activity was inactivated about 75% by 1mM of o-phenanthroline and monoiodoacetate. But the enzyme activity was stimulated up to 200% by 1mM of 2-mercaptoethanol. | ||
| Download PDF | Kor_260413_368-374p.pdf | ||