Title 감자 걀쪽바이로드(PSTV) RNA 분자의 요소농도기울기겔에서 전기영동적 이동성에 관하여
Author 이재열
Address 경북대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 25(1),23-27, 1987
DOI
Key Words viroid-RNA molecules, urea-gradient polyacrylamide gel
Abstract Low molecular weight plant ribonucleic acids including viroid-RNA molecules which are soluble in 2M lithium chloride were electrophoresed in the 0M to 8M urea-gradient polyacrylamide gel. Although the linear viroid-RNA molecules migrated at a similarrate across the urea-gradient gel under the denaturing temperature, the circular viroid-RNA molecules moved more rapidly at low urea-gradient region than at high urea-gradient region. Consequently, the migration of the circular viroid-RNA molecules showed a sudden shift across the band of linear forms in the midrange of the urea-gradient gels. Electrophoretic mobilities of the circular viroid-RNA molecules seemed to depend mainly on the concentration of urea in the denaturing urea-gradient gels.
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