Title |
감자 걀쪽바이로드(PSTV) RNA 분자의 요소농도기울기겔에서 전기영동적 이동성에 관하여 |
Author |
이재열 |
Address |
경북대학교 자연과학대학 미생물학과 |
Bibliography |
Korean Journal of Microbiology, 25(1),23-27, 1987 |
DOI |
|
Key Words |
viroid-RNA molecules, urea-gradient polyacrylamide gel |
Abstract |
Low molecular weight plant ribonucleic acids including viroid-RNA molecules which are soluble in 2M lithium chloride were electrophoresed in the 0M to 8M urea-gradient polyacrylamide gel. Although the linear viroid-RNA molecules migrated at a similarrate across the urea-gradient gel under the denaturing temperature, the circular viroid-RNA molecules moved more rapidly at low urea-gradient region than at high urea-gradient region. Consequently, the migration of the circular viroid-RNA molecules showed a sudden shift across the band of linear forms in the midrange of the urea-gradient gels. Electrophoretic mobilities of the circular viroid-RNA molecules seemed to depend mainly on the concentration of urea in the denaturing urea-gradient gels. |
Download PDF |
Kor_250104_23-27p.pdf |