| Title | 알킬화제 시약에 대해 민감한 E. coli 변종들과 그들의 상보적인 유전자에 대한 연구 | ||
| Author | 정선호 · 한범희 · 양철학 | ||
| Address | 서울대학교 자연과학대학 화학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(1),57-65, 1987 | ||
| DOI | |||
| Key Words | MNNG mutagenesis, MMS-sensitive mutant, Host-Cell Reactivation, Recombinant plasmid pMRG 1 | ||
| Abstract | Mutants of E. coli which showed increased sensitivity to MMS(methylmethane sulfonate)were isolated by MNNG mutagenesis and characterized by enzymatic assay, survival of simple alkylating agents and host-cell reactivation. E.coli mutant, 5-62, which showed absolute deficiency in 3-methyladenine DNA glycosylase II activity and had low capability of reactivating MMS-treated phage charon 35 was very sensitive to MMS and MNNG. NNS gene which confered resistance to the lethal effects of MMS was cloned in 5-62 strain. 5-62 mutants carrying recombinant plasmid, pMRG 1, which acquired resistance to the lethal effects of MMS had normal sensitivity to MNNG. Resistance to MMS was somewhat increased after they were treated with 0.5㎍ MNNG/ml for 2 hours at 37℃. Although recombinant plasmid, pMRG 1, did not complement alk A mutation in 5-62 and ada mutation in 1-27 mutnat, mutnats transformed with this plasmid showed more capability of reactivating MMS treated phage than mutants. | ||
| Download PDF | Kor_250110_57-65p1.pdf | ||