| Title | Klebsiella pneumonia로 부터 제한효소 Kpn I의 분리 | ||
| Author | 이상철 · 이대실 · 유명희 | ||
| Address | 한국과학기술원 유전공학센터 | ||
| Bibliography | Korean Journal of Microbiology, 25(1),73-79, 1987 | ||
| DOI | |||
| Key Words | Kpn I, restriction endonuclease, ligation-recut test | ||
| Abstract | A restriction endonuclease, Kpn I has been isolated from Klebsiella pneumonia. Cells were broken by sonication. After ultracentrifugation the supernatant containing Kpn I activity was further purified by Sepharose-6B gel filtration, DEAD-Cellulose, Heparin-Agarose, and Aminohexyl-Agarose column chromatography. Final enzyme preparation was essentially free of contamination exonuclease and phosphatase, as judged by ligation-recut test. Total activity of the enzyme recovered from 10 grams of cells was 4.7×10^5 units. | ||
| Download PDF | Kor_250112_73-79p.pdf | ||