| Title | 염화 방향족 탄화수소 분해세균의 분리 및 특성 | ||
| Author | 김종우 · 김치경 · 김영창 · 염재홍 · 이재구 * | ||
| Address | 충북대학교 자연대 미생물학과; * 농대 농화학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(2),122-128, 1987 | ||
| DOI | |||
| Key Words | chlorinated aromatic hydrocarbon-degrading bacteria, 2,4,5-trichlorophenoxyacetic acid, 4-chlorobiphenyl | ||
| Abstract | Several bacterial isolates capable of degrading 4-chlorobiphenyl or 2,4,5-trichlorophenoxyacetic acid were isolated from industrial wastes by the agar plate method and studied for their biodegradabilities of the hydrocarbons and some biochemical characteristics. The isolates DJ-12, DJ-26 and TP-1 were identified as Pseudomonas spp. and they could not degrade 2,4-dichlorophenoxyacetic acid. The absorption spectra for 4-chlorobiphenyl and 2,4,5-trichlorophenoxyacetic acid showed the peaks at 253 and 292 nm, respectively. Biodegradability of the isolates was determined by decrease of the absorbance for the test hydrocarbons with a UV-scanning spectrophotometer. The plasmids of the isolates were studied to examine whether or not the hydrocarbon-degrading genes exist in the plasmids. Antibiotics resistance was also examined to search out a proper marker for the isolates in further experiments, such as curing test and genetic recombination. | ||
| Download PDF | Kor_250206_122-128p.pdf | ||