| Title | 고온성 clostridium thermocellum이 생산하는 Endo-β-1, 4-glucanase의 정제 및 성질 | ||
| Author | 김욱한 · 하지홍 · 정기택 * · 이용현 | ||
| Address | 경북대학교 자연과학대학 유전공학과; * 농과대학 식품공학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(2),157-164, 1987 | ||
| DOI | |||
| Key Words | Clostridium thermocellum, endo-β-1, 4-glucanase, purification, enzyme properties | ||
| Abstract | A new endo-β-1, 4-glucanase was purified from the culture filtrate of thermophilic anaerobic Clostridium thermocellum. The purification procedure included two steps of ion exchange chromatography with DEAD-Sephadex A-50 and gel filtration chromatography with Sephadex G-75. Even though the 56 fold increase in CMCase specific activity was obtained, the actually recovered enzyme activity was relatively lower level of 0.7%. Judging from the two bands in SDS-polyacrylamide gel electrophoresis, the endo-β-1, 4-glucanase consists of two subunits whose M.W. are 38,000 and 58,000, respectively. The optimum pH and temperature were determined to be 5.0 and 65℃, respectively. The enzyme was stable up to 70℃, but inactivated at 80℃. The kinetic parameters of the separated fraction were also determined. The purified enzyme did not show any significant hydrolytic activity against the highly ordered crystalline cellulose as well as filter paper. | ||
| Download PDF | Kor_250211_157-164p.pdf | ||