| Title | Streptomyces tubercidicus에 존재하는 stu I endonuclease의 정제와 특징 | ||
| Author | 김기태 · 정미영 · 유욱준 | ||
| Address | 한국과학기술원 생물공학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(3),180-183, 1987 | ||
| DOI | |||
| Key Words | Type II restriction endonuclease, Stu I, homogenous preparation | ||
| Abstract | Stu I, a type II restriction endonuclease, has been purified to homogeneity from Streptomyces tubercidicus (ATCC 25502), and its catalytic properties have been studied. For the purification of Stu I endonuclease free of nonspecific nucleases, DEAE-Sephadex (A-50), QAE-Sephadex (A-50) and Heparin-agarose column chromatography have been performed after ammonium sulfate fractionation of the crude extract. The enzyme was further purified by gel filtration using Sephadex G-100 column to obtain homogeneous form of protein. The single polypeptide species of Stu I endonuclease has a subunit molecular weight of 34,000±1,000 daltons as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Stu I endonuclease requires Mg^2+ ion for its activity and is maximally active at neutral pH (7.0-8.0) in the absence of NaCl. | ||
| Download PDF | Kor_250303_180-183p.pdf | ||