| Title | Streptomyces fradiae에서 대사중간산물 이용속도에 의한 균체 성장과 tylosin 생합성의 조절 | ||
| Author | 강현아 · 이계준 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(3),189-197, 1987 | ||
| DOI | |||
| Key Words | Streptomyces fradiae, tylosin, secondary metabolite, tylactone, metabolic regulation | ||
| Abstract | The aim of the present study was to investigate the effect of glutamate on the biosynthesis of tylosin. Activities of enzymes involved in the metabolic pathway of glutamate to form tylactone, an essential precursor of tylosin, were determined using Streptomyces fradiae grown at different concentration of glutamate. As results, it was found that cell growth and tylactone formation was controlled by the metabolic flux of oxaloacetate. It was clear that cell growth was favored by the activities of citrate synthase and aspartate aminotransferase, while the tylactone synthesis was stimulated by the activity of methylmalonyl-CoA carboxyltransferase. Therefore it was concluded that channelling of oxaloacetate was a point for favoring either cell growth or tylosin biosynthesis. | ||
| Download PDF | Kor_250305_189-197p.pdf | ||