| Title | 원형질체 융합에 의한 pseudomonas putida의 biparental clones의 형성과 성질 | ||
| Author | 이주실 · 이영원 · 이영록 | ||
| Address | 고려대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(3),198-204, 1987 | ||
| DOI | |||
| Key Words | Pseudomonas putida, spheroplast fusion, biparental clone | ||
| Abstract | Biparental clones and recombinant clones were obtained by spheroplast fusion of Pseudomonas putida KU218R-3 and P.putida KU428. Formation of the fusion product was the most effective when the Pseudomonas spheroplast mixture were treated with 40% plyethyleneglycol(PEG) 6000 for 10min at room temperature, The fusants which selected by indirect method were obtained at an average frequency of 10.8%. Most of the fusants were biparental clones (10.4%) and the recombinant clones were produced in low yield (0.42%). Fusants, at the frequency of 4% were obtained without PEG 6000, which shows that fusion is not strictly dependant on PEG. The stability of fusants were examined. Most of the biparental clones were segregated to parental form and late recombinants were formed on further propagation of biparental clone but the recombinant clones were very stable. | ||
| Download PDF | Kor_250306_198-204p.pdf | ||