| Title | 메치오닌 유사체 내성 cephalosporium acremonium 변이주의 특성(Characterization of methionine analogue-resistant mutant of cephalosporium acremonium) | ||
| Author | 이경 · 최상호 · 이정준 · 민태익 | ||
| Address | 한국과학기술원 유전공학센터 미생물공학 연구실 | ||
| Bibliography | Korean Journal of Microbiology, 25(3),205-211, 1987 | ||
| DOI | |||
| Key Words | cephalosporium acremonium, cephalosporin C(CPC), DL-seleno-menthionine, sulfate uptake, L-methionine uptake | ||
| Abstract | Cephalosporium acremonium MAR-80, a strain of methionine analogue-resistant mutant, showed good activity of sulfate utilization as only sulfur source. The effect of methionine on the sulfate uptake system was investigated by using Na_2^35SO_4 as a tracer in the resting cell system. From this result, it was revealed that sulfate permease of this strain was less repressed and/or less inhibited by methionine than parent type. This deregulation was due to low activity of methionine uptake, which was operated by somewhat simple diffusion. From these studies, it could by anticipated that the improved productivity of cephalosporin C and lower dependence of cephalosporin C production on methionine were related to increased uptake rate of sulfate. | ||
| Download PDF | Kor_250307_205-211p.pdf | ||