| Title | 수정된 gram 염색법에 의한 혼합세균 개체군의 분별 측정 | ||
| Author | 장진경 · 임종락 · 정계효 * · 한홍의 | ||
| Address | 인하대학교 이과대학 생물학과; * 서울기독병원 | ||
| Bibliography | Korean Journal of Microbiology, 25(3),244-248, 1987 | ||
| DOI | |||
| Key Words | Gram stain, cell turbidity, Micrococcus luteus, Escherichia coli, hemp, Kimchi | ||
| Abstract | Attempts were made to enumerate the number of Gram positive and negative bacteria in the development of natural fermentation rapidly and simultaneously. A general Gram stain was applied to this study. The number of cells by Gram stain was proportional to the cell turbidity by spectrophotometer within a range of 0.7 absorbance at 610nm. The cells washed out during procedures were not exceeded about 8 percentage. The standard error of separate counts in the mixture of Escherichia coli and Micrococcus luteus was 5.1±2.3%. The possible range of counting was 5.5×10^7 - 1.0×10^9 cells/ml. Therefore, it is believed that a general Gram stain could be applied to the separate counting of mixture of Gram positive and negative bacterial populations too. In practice, growth kinetics of hemp retting and Kimchi fermentation were presented. | ||
| Download PDF | Kor_250312_244-248p.pdf | ||