| Title | 교류 펄스에 의한 saccharomyces cerevisiae의 생장억제 효과 | ||
| Author | 정지환 · 박현근 · 한홍의 | ||
| Address | 인하대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(3),249-253, 1987 | ||
| DOI | |||
| Key Words | AC pulse, Saccharomyces cerevisiae, growth inhibition, lethality, auxotroph | ||
| Abstract | Effects of Ac pulse at low voltage on Saccharomyces cerevisiae were studied. The treatment of yeast suspensions contained 0.2m NaCl with 500mA for 350 sec at 40℃ was shown about 50% of lethality, whereas in the treatment of the same suspensions with 250mA for 250 sec at the temperature (10℃) corresponding to about 10% of lethality, growth was completely inhibited instead of lethality. The effect of growth inhibition was due to occurrence of auxotrophic strains under experimental conditions. Detection of auxotrophic yeasts was done tentatively with the difference of the number of viable yeast cells between direct counting by methylene blue staining and plate-count on yeast morphology agar. | ||
| Download PDF | Kor_250313_249-253p.pdf | ||