| Title | Bovine serum albumin, myoinositol과 ergosterol에 의한 candida pseudotropicalis의 원형질체 재생 및 융합증진 | ||
| Author | 전순배 · 배석 | ||
| Address | 전남대학교 자연과학대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(4),274-281, 1987 | ||
| DOI | |||
| Key Words | Candida pseudotropicalis, protoplast regeneration and fusion | ||
| Abstract | The effects of bovine serum albumin, myoinositol and ergosterol on protoplast formation, regeneration and fusion from auxotrophic mutants of Candida pseudotropicalis were examined. Frequency of protoplast formation ranged from 48 to 98% depending on auxotrophic types. When myoinositol (0.5mg/ml) and ergosterol (0.1mg/ml) were supplemented in the medium of cell growth, and bovine serum albumin (4mg/ml)was added to protoplasting buffer, 50-100% of cells were converted to protoplasts. Such a treatment of three additives improved 2.2-3.0 fold of regeneration rate of protoplasts. The fusion frequencies between complementary auxotrophs ranged from 7.0×10^-4 to 1.5×10^-3 in the optimal conditions. These values showed 1.9-2.3 fold increase when compared with fusion frequencies obtained without the treatment of additives. These results suggested that these compounds may improve protoplast regeneration and fusion between complementary auxotrophs used in this study. | ||
| Download PDF | Kor_250403_274-281p.pdf | ||