| Title | 섬유소 분해시 혐기성 clostridium thermocellum이 생산하는 cellulase의 C₁성분의 역할과 성질 | ||
| Author | 이용현 · 김욱한 · 신현동 | ||
| Address | 경북대학교 자연과학대학 유전공학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(4),297-303, 1987 | ||
| DOI | |||
| Key Words | Clostridium thermocellum, C₁component, Cellulose degfadation | ||
| Abstract | A C₁enriched cellulase fraction was separated from culture filtrate of anaerobic Clostridium thermocellum by hydroxyapatite column chromatography. The separated fraction showed strong synergistic action with C_x component (endo-β-1, 4-glucanase) in digestion of crystalline cellulose, similar to the other aerobic cellulolytic microorganisms. Unlike the C_x component the C₁ enriched fraction was rapidly inactivated by oxidation at the atmospheric condition. The enzyme activity was significantly enhanced by the addition of reducing agents, especially β-mercaptoethanol, which indicates that a C₁ component has a lot of sulfhydryl groups essential for the enzyme activity. The effect of metal ions on C₁ activity was also investigated. The C₁ fraction was found to be thermally stable compare to endo-β-1,4-glucanase. Optimal temperature and pH were found to be 60× and 6.0, respectively. | ||
| Download PDF | Kor_250406_297-303p.pdf | ||