| Title | 면역유충(Lucilia illustris) 체액으로 부터 항균물질의 유도 | ||
| Author | 육순학 · 장정순 | ||
| Address | 인하대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(4),309-317, 1987 | ||
| DOI | |||
| Key Words | Bactericidal Sucstance, larval haemolymph, Lucilia illustris | ||
| Abstract | It was known that normal-haemolymph from the 3rd instar larvae of Lucillia illustris contain a lysozyme (or lysozyme-like substance) with bactericidal activity to gram positive bacteria, and the bactericidal activity of injured-haemolymph was increased significantly after injuring the body wall. To elucidate the defence mechanism of insect against the nonpathogenic bacteria, the immune-haemolymph against Escherichia coli K-12 was prepared after immunization. The bactericidal activity between injured and immune-haemolymph was compared, and it was revealed that the immune-haemolymph showed higher titer of bactericidal activity to gram positive bacteria as well as to Escherichia coli. The bactericidal substance from the immune-haemolymph was purified through a successive chromatographies on Sephacryl S-300 and CM-Sepharose CL-6B, and it was characterized as a basic protein in nature with heat stable property at acidic conditions. | ||
| Download PDF | Kor_250408_309-317p.pdf | ||