| Title | Streptomyces fradiae에서 균 성장속도에 의한 tylosin 생합성 조절 | ||
| Author | 강현아 · 이정현 · 이계준 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 25(4),353-359, 1987 | ||
| DOI | |||
| Key Words | Streptomyces fradiae, tylosin, secondary metabolite, metabolic regulation | ||
| Abstract | The aim of the present study was to investigate the effects of growth rate on the biosynthesis of tylosin in Streptomyces fradiae. In order to elucidate the relation between the growth rate and the tylosin formation rate, the activities of enzymes involved in oxaloacetate metabolism were determined using cells grown at different growth rates in chemostats. As the results, it was found that the specific rate of tylosin formation(q_p) was closely related to the specific cell growth rate and the maximum value of q_p was 1.1mg tylosin, g^-1 cell, h^-1 at the growth rate 0.013h^-1. However further increase in the growth rate over 0.013h^-1 resulted in apparent decrease of q_p. The synthesis and activities of citrate synthase, aspartate aminotransferase, and PEP carboxylase were very low at lower growth rate. On the other hand, the activity and synthesis of methylmalonyl-CoA carboxyltransferase was closely related to tylosin formation. Therefore it was concluded that tylosin formation was apparently controlled by the growth rate. | ||
| Download PDF | Kor_250414_353-359p.pdf | ||