| Title | 제한효소 Sma I, Xma I, AVa I, Nae I의 DNA절단반응에 있어서 Hpa II methylation의 억제효과 | ||
| Author | 최우성 · 강선철 · 서정선 * · 유욱준 | ||
| Address | 한국과학기술원 생물공학과; * 서울대학교 의과대학 생화학교실 및 암연구소 | ||
| Bibliography | Korean Journal of Microbiology, 24(2),86-90, 1986 | ||
| DOI | |||
| Key Words | restiriction modification engyme, DNA protein interaction | ||
| Abstract | The DNA methylated by Hpa II methylase was not cleaved by Sma, I, Ava I and Nae I endonucleases. This experimental data could be interpreted as strong evidences that Sma I, Ava I and Nae I methylases which yet to be isolated would methylate on the inmost cytosine nucleotide within their hexameric recognition sequences. The facts that Sma I, Ava I and Nae I endonucleases can not cleave the DNA methylated by Hpa II methylase are the valuable informations for protecting DNAs upon cleavage reactions by Sma I, Ava I and NAe I endonucleases especially for cDNA insertion experiments into vector DNAs using Sma I, Ava I and Nae I oligonucleotide linkers. In the case of Xma I endonuclease, partially cleaved DNA fragments were observed although the reaction rate was greatly decreased. This result implies that the methylation site of Xma I methylase which yet to be isolated would not be the same as that of Hpa II methylase in Xma I sequence. | ||
| Download PDF | Kor_240202_86-90p.pdf | ||