| Title | Trichoderma koningii와 trichoderma reesei 원형질체 융합 | ||
| Author | 박희문 · 정종문 · 홍순우 · 하영칠 · 성치남 ** | ||
| Address | 서울대학교 자연과학대학 미생물학과; ** 순천대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 24(2),91-97, 1986 | ||
| DOI | |||
| Key Words | protoplast fusion, Trichoderma koningii, Trichoderma reesei, intespecific hybrid | ||
| Abstract | Intra and interspecfic fusants were produced by the protoplast fusion of auxotrophic mutants from Trichoderma koningii ATCC 26113 and Trichoderma reesei QM 9414. It was found that 0.6M MgSO₄and 0.6M NH₄Cl was the best osmotic stabilizer for the preparation of protoplasts from the mycelium of T. koningii and T. reesei respectively. However, MgSO₄was the most suitable one for the regeneration of the protoplasts from both species. The intraspecific protoplast fusion frequencies between the auxotrophic mutants from T. reesei were 1.8×10^-2 to 5.1×10^-1. Interspecific protoplast fusion frequencies between the auxotrophic mutants from T. koningii and T. reesei were 3.6×10^-3 to 8.4×10^-2. Interspecific complementing fusants, however, were not always produced. Fusants obtained from interspecific potoplast fusion were spontaneously segregated into various strains including parental types, non-parental auxotrophic hybrids, and prototrophic hybrids on complete plate. Interspecific hybrids revealed to have partially enhanced celluloytic activities. | ||
| Download PDF | Kor_240203_91-97p0.pdf | ||