Title Lentinus edodes JA01에서 분리한 세포외 polyphenol oxidase의 부분적 성질에 관하여
Author 신광수 · 김규중 * · 홍순우 · 하영칠
Address 서울대학교 자연과학대학 미생물학과; 강릉대학 생물학과 *
Bibliography Korean Journal of Microbiology, 24(2),141-146, 1986
DOI
Key Words lignosulfonate-NH₄salts, polyphenol oxidase, Lentinus edodes
Abstract To find the role of polyphenol oxidase in lignin biodegradation, charcteristics of extracellular polyphenol oxidase activity from Lentinus edodes JA01 was investigated. Polyphenol oxidase had its optimum activity at pH 4.5 and 45℃ respectively. Also, the enzyme was very unstable in various pHs and comparatively heat stable up to 60℃. In lignosulfonate-NH₄salts medium, the growth rate of L.edodes JA 01 was relatively slow and polyphenol oxidase activity appeared 2 and 14 days after inoculation. No significant relationships were found between polyphenol oxidase activity and the amounts of lignosulfonate present in the culture medium.
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