| Title | Streptomyces levendulae의 원형질체와 원형질체 융합에 대한 미세구조 | ||
| Author | 하영칠 · 홍순우 · 유진철 · 임헌만 * | ||
| Address | 서울대학교 자연과학대학 미생물학과; * 연세대학교 의과대학 | ||
| Bibliography | Korean Journal of Microbiology, 24(3),197-203, 1986 | ||
| DOI | |||
| Key Words | Streptomyces levendulae, Protoplast fusion, ultrastructure | ||
| Abstract | Morphology and ultrastructure of protoplast fusion mode in Streptomyces lavendulae were studied by scanning and transmission electron microscopy. The isolated protoplasts were stable in some degree in hypertonic solution except that several protoplasts showed irregular morphology. Fusion events were occurred as follows; contact zone, fusion zone and separation zone were appeared sequentially. After formation of the separation zone, cytoplasm and DNA from both parents were mixed eventually. In the contact zone, two menbranes were still separated by electron transparent space. The contact zone changed to fusion zone by formation of fusion membrane that phospholipid molecules of two membranes were rearranged. Thereafter, nonmembraneous separation zone was formed by disappearance of fusion membrane. These changes were characterized by successive changes in typical membrane structure in fusion areas and by a progressive loss of bispherical shape. | ||
| Download PDF | Kor_240301_197-203p.pdf | ||