| Title | Bacillus subtilis로 부터 분리한 cellulase 유전자의 조절부위에 대한 염기서열분석 | ||
| Author | 서연수 · 이영호 · 백운화 · 강현삼 * | ||
| Address | 두산연구소 유전공학연구실; * 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 24(3),236-242, 1986 | ||
| DOI | |||
| Key Words | Bacillus subtilis, cellulase, DNA sequencing, signal seuence | ||
| Abstract | The nucleotide sequence of the genetic control site of Bacillus subtilis gene for (1-4)-β-D-glucan endoglucanase (cellulase) was determined according to the procedures of the dideoxy chain termination method(Sanger et. al., 1977). The deduced amino acid sequence of this enzyme has a hydrophobic signal peptide at the NH₂terminus similar to those found in fifteen other extracellualr enzymes from Bacillus species. This is followed by a sequence resembling the Bacillus ribosome binding site 14 nucleotide before the first codon of the gene. The presumptive promoter sequence was located 92 base pairs upstream fromthe initiation codon. The homology region in signal sequences was striking when comparing all the signal sequences of sixteen extracellular enzymes from Bacillus species so far compiled. | ||
| Download PDF | Kor_240306_236-242p.pdf | ||