| Title | Escherichia coli K12의 막단백질 형성과 페니실린 민감성에 대한 산소의 능동적 역할 | ||
| Author | 박현근 · 한홍의 | ||
| Address | 인하대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 24(3),263-269, 1986 | ||
| DOI | |||
| Key Words | aerobiosis, anaerobiosis, membrane protein, penicilllin sensitivity | ||
| Abstract | Membrane proteins of facultatively anaerobic Escherichia coli K12 which was logarithmically grown in aerobiosis and anaerobiosis were compared on 5 to 10% liner gradient gel electrophoresis (Na Dod SO₄-PAGE). Membrane proteins were formed as different patterns between aerobiosis and anaerobiosis. Among them, 91Kdal protein (pbp1a) was not synthesized in aerobiosis and 60Kdal protein (fts cluster), in anaerobiosis. Thereby cells cultured aerobically were differenciated as diversiform cell shape, comparing cells cultured anaerobically and the latter were resistant to penicillin G. Thus it is believed that in facultative anaerobes atmospheric oxygen regulated the synthesis of membrane proteins and even the expression of equivalent genes, and moreover alleviated the resistance to an antibiotic penicillin. | ||
| Download PDF | Kor_240309_263-269p.pdf | ||