| Title | Pseudomonas carboxydovorans와 acinetobacter sp.1의 일산화탄소 산화효소의 세포내 분포에 대한 효소학적 연구 | ||
| Author | 김시욱1 · 김영민 * | ||
| Address | 연세대학교 이과대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 24(3),270-275, 1986 | ||
| DOI | |||
| Key Words | Pseudomonas carboxydovorans, Acinetobacter, CO dehydrogenase | ||
| Abstract | The localization of carbon monoxide dehydrogenases (CO-DHs) in Pseudomonas carvoxydovorans and Acinetobacter sp.1 was examined by comparison of the distribution of CO-oxidizing activity between soluble and particulate fractions obtained after disruption of CO-grown cells by sonic oscillation and of spheroplasts by osmotic shock. When the cells were broken by sonic oscillation, most of the CO-DH activity was recovered from soluble fractions. However, disryption by osmotic lysis of spheroplasts revealed that the enzyme activity is present in the cell membrane. The results indicated the CO-DHs in these cells are loosely attached to the cytoplasmic membrane. | ||
| Download PDF | Kor_240310_270-275p.pdf | ||