| Title | β-1, 3-glucanase 생성균의 분리 및 효소 생성 조건 | ||
| Author | 정기택 · 방광웅 · 송형익 · 김재근 · 유대식 * | ||
| Address | 경북대학교 식품가공학과; * 계명대학교 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 24(3),295-301, 1986 | ||
| DOI | |||
| Key Words | β-1, 3-glucanase, Pseudomonas stutzeri KF 13, Cell wall | ||
| Abstract | The bacteria, which were capable of producing β-1, 3-glucanase inducibly by utilizing cell wall of Aspergillus fumigatus as a sole carbon source, were isolated from soil in the campus of Kyungpook National University. Among them, the strain which produced the enzyme excellently was selected and identified to be Pseudomonas stutzeri KF 13 by morphological, cultural and physiological examination. The optimal conditions for the enzyme production from Pseudomonas stutzeri KF 13 were investigated. The enzyme production was reached maximum state when the broth cultured for 72hr at 30℃. And the enzyme showed the highest activity in the medium containing 3.5% cell wall as an inducer, 15% yeast autolysate as a nitrogen source and 0.05% MnSO₄at pH 7.5. | ||
| Download PDF | Kor_240313_295-301p.pdf | ||