Title Staphylococcus aureus에서 분리된 plasmid상의 항생물질 저항성 인자에 관한 연구 : chloramphenicol 저항성 인자의 클로닝
Author 권동현 · 김영선 · 변우현
Address 강원대학교 자연과학대학 생물학과
Bibliography Korean Journal of Microbiology, 24(4),341-351, 1986
Key Words Chloramphenicol resistance, Staphylococcus aureus
Abstract R-plasmid(pSBK203, 2.5Mdal) conferring chloramphenicol resistance was isolated from mutiple antibiotic resistant Staphylococcus aureus D-H-1. Bacillus subtilis BD170 was transformed by this plasmid and restriction enzyme clevage sites of this plasmid were mapped for the cloning of chloramphenicol resistance gene. Taq I partial digested fragment of pSBK203(1.3kb) inserted into Cla I site of pBD9 appears to have both regulatory region for induction and structural gene for chloramphenicol resistance whereas Rsa I fragment (1.3kb, both ends are staggered away 0.1Kb from those of Taq I fragment) inserted into Sca I site of pBR322 showed constitutive expression in E. coli. Hinf I, Taq I, and Bgl II restriction enzyme recognition sites are found in both Rsa I fragment and Taq I fragment. Among these, Bgl II recognition site was associated with chloramphenicol resistance.
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