Title |
새로운 type II 제한효소 Xci I의 분리 |
Author |
황영 · 채건상 · 장원희 · 김기태 · 유욱준 |
Address |
한국과학기술원 생물공학과 |
Bibliography |
Korean Journal of Microbiology, 24(4),406-410, 1986 |
DOI |
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Key Words |
type II endonuclease, Xci I, isoschizomer, Sal I, cleavage site |
Abstract |
The isolation and charateriation of a type II restriction endonuclease from Xanthomonas citri IFO 3835 were described. This enzyme (Xci I endonuclease) is an isoschizomer of Sal I endonuclease recognizing 5'-GTCGAC-3' and cleaving at the site indicated by the arrow. Unlike Sal I endonuclease, Xci I endonuclease requries a NaCl concentration of 50mM for its maximum activity. |
Download PDF |
Kor_240412_406-410p.pdf |