Title 새로운 type II 제한효소 Xci I의 분리
Author 황영 · 채건상 · 장원희 · 김기태 · 유욱준
Address 한국과학기술원 생물공학과
Bibliography Korean Journal of Microbiology, 24(4),406-410, 1986
Key Words type II endonuclease, Xci I, isoschizomer, Sal I, cleavage site
Abstract The isolation and charateriation of a type II restriction endonuclease from Xanthomonas citri IFO 3835 were described. This enzyme (Xci I endonuclease) is an isoschizomer of Sal I endonuclease recognizing 5'-GTCGAC-3' and cleaving at the site indicated by the arrow. Unlike Sal I endonuclease, Xci I endonuclease requries a NaCl concentration of 50mM for its maximum activity.
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