| Abstract |
The aim of this investigation was to resolve the contradiction between the results of Sternberg and Mandels (1980, 1982)and those of Nisizawa et al., (1971) in cellulase induction by sophorose, and furthermore to study the conditional effects in sophorose-induced cellulase induction in Trichoderma reesei QM 9414. Sophorose could induce the synthesis of CMCase and β-glucosidase simultaneously. Optimal induction medium by sophorose had the potassium citrate buffer solution of pH 3.0-4.0 for CMCase, but one of pH 5.0-6.0 for β-flucosidase. At this time, two different types of β-glucosidases could be induced by sophorose: one was extracellular and had maximum at pH 5.0, the other was intracellular and had maximum activity at pH6.5. Induction study showed that methyl-β-glucoside was not a true inducer of β-glucosidase and that large β-glucosidase induction could be obtained only by the addition of sophorose into the induction medium. Glucose repressed the induction of cellulase by sophorose. The repression of glucose could not be overcome by the addition of cyclic AMP into the induction medium. |
