| Abstract |
Five representative virulent phages (J1, TK93, K1, PD5, and CP1) and one temperate phage (φ1043) of Lactobacillus casei were compared to each other by analyzing the agarose gel electrophoretic patterns of restriction enzyme-digested phage DNAs. Nucleic acids of all the tested phages were double stranded DNA. DNAs of J1, TK93, K1, and φ1043 phages had a size of about 42kb, but the size of PD5 and CP1 DNAs was about 140kb. J1, TK93, K1, PD5, CP1, and φ1043 DNAs were digested to 13, 13, 11, 14, 14, and 12 fragments by EcoR1, respectively, and showed its characteristec restriction patterns. Cohesive ends were present in J1, TK93, and φ1043, but were absent in K1, PD5, and CP1. Restriction maps of J1 and TK93 DNAs showed nearly complete homology and their evolutionary relationship based upon the restriction analysis was discussed. |
