| Title | Ashbua gossypii에서의 리보플라빈 측쇄의 기원 | ||
| Author | 최원자 · 임정빈 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 23(3),167-171, 1985 | ||
| DOI | |||
| Key Words | The origin of ribityl side chain of riboflavin in ashbya gossypii | ||
| Abstract | In order to investigate the origin of the ribityl froup of riboflavin and the involvement of GTP cyclohydrolase II in the riboflavin pathway, we studied the incorporation of ¹⁴C-labeled guanosine using a well known riboflavin over producer, Ashbya gossypii.Cells were grown in a media containing (U-¹⁴C) guanosine and the riboflavin and GMP were isolated and purifired by column chromatography. The isolated compounds, riboflavin and GMP were labeled in the ribityl and ribosyl side chain and the isoalloxazine and guannine moiety. By comparing the specific radioactivity of each compound we reached a conclusion that the ribose of guanosine is converted directly to the ribityl moiety of riboflavin. The results indicate that biosynthesis of the vitamin begins at the level of a guanosine compound and also support the involvement of GTP cyclohydrolase II in one of the early steps in the biosynthetic pathway. | ||
| Download PDF | Kor_230301_167-171p.pdf | ||