| Title | Rhizobium japonicum원형질체 형성과 근류 bacteroids에 미치는 glycine의 영향 | ||
| Author | 김성훈 · 김창진 · 이윤 · 유익동 · 민태익 | ||
| Address | 한국과학기술원 유전공학센터 | ||
| Bibliography | Korean Journal of Microbiology, 23(3),197-202, 1985 | ||
| DOI | |||
| Key Words | Glycine effect on spheroplasting and nodule bacteroids of rhizobium japonicum | ||
| Abstract | Different spheroplasting methods using glycine were tried to fast and slow-growing R. japonicum. Although one of the fast growers, R-271 showed normal growth in the presence of 4mg/ml glycine, cell morphology and colony forming unit (CFU) were greatly different from the cells of late log phase grown in the medium without glycine. In parallel, R-271 became sensitive to lysozyme after 6hr incubation in medium containing glycine (3.5mg/ml). After 24hr cultivation in glycine (100ug/ml) medium, one of the slow growers, R-214 was also susceptible to lysozyme action. Spgeroplasting frequency of both strains was over 96% by glycine and lysozyme. Spheroid cell was also found in bacteroids from root nodule and soluble glycine content was relatively smaller than other amino acids in soybean nodule extracts. | ||
| Download PDF | Kor_230306_197-202p.pdf | ||