| Title | 원형질체 융합법에 의한 cellulomonas sp. CS 1-1의 유전자 재조합 | ||
| Author | 김병홍 · 이학주 | ||
| Address | 한국과학기술원 생물공학부 미생물연구실 | ||
| Bibliography | Korean Journal of Microbiology, 23(4),309-314, 1985 | ||
| DOI | |||
| Key Words | Genetic Recombination by Protoplast Fusion of Cellulomonas sp CS 1-1 | ||
| Abstract | Autoxotrophic mutants of a cellulolytic baterium Cellulomonas sp. CS 1-1 were grown at 30℃ for 6hr using a complete medium containing 0.5M sucrose and for another 90 min after addition of 0.3 U/ml penicillin G, and were protoplasted by 0.2mg/ml lysozyme for 2hr. Prototrophic recombinants were obtained at the rates of 10^-3 to 10^-5 by fusing the protoplasts in the presence of 40% polyethyleneglycol3350. Nystatin could be used to eliminate fungal contamination during the regeneration of the plotaplasts. | ||
| Download PDF | Kor_230411_309-314p.pdf | ||