| Title | Novozym 234에 의한 Kluyveromyces fragilis N100과 Candida pseudotropicalis CBS607의 원형질체 형성과 재생 | ||
| Author | 배석 · 전순배 | ||
| Address | 전남대학교 자연과학대학 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 22(1),49-56, 1984 | ||
| DOI | |||
| Key Words | Formation and Regeneration of protoplasts by Novozym 234 from Kluyveromyces fragilis N100 and Candida pseudotropicalis CBS607 | ||
| Abstract | Formation and regeneration of protoplasts by Novozym 234 from Kluyveromyces fragilis N100 and Candida pseudotropicalis CBS607 were studied. This enzyme was more effective on cells grown at exponential phase than those at stationary one to convert intact cells into protoplasts. As osmotic stabilizer, ammonium sulphate was suitable for not only protoplast formation but also regeneration in K. fragilis as well as in C. pseudotropicalis. Optimal enzyme concentration was 3mg per ml in K. fragilis and 1~3mg per ml in C. pseudotropicalis, respectively. After the exposure of K. fragilis cells to 3mg per ml of enzyme for 3hr at 30℃, approximately 95% of protoplast formation of all observed cells was obgained, while about 100% from C. pseudotropicalis under the same condition was produced. The regeneration frequency of protoplasts by this enzyme was much lower than that by snail enzyme(Glusulase) although Novozym 234 converted cells from above two species into protoplasts free of cell debris effectively, compared with Glusulase. Novozym 234 appears to be suitable for subcellular fractionation to obtain nuclei or other organelles rather than protoplast regeneration. | ||
| Download PDF | Kor_220106_49-56p.pdf | ||