Title Candida kefyr의 β-D-galactosidase 合成誘導에 關한 硏究[I]
Author 全順培
Address 全南大學校 自然科學大學 生物學科
Bibliography Korean Journal of Microbiology, 22(2),77-84, 1984
Key Words Studies On Induction of β-D-galactosidase In Candida kefyr
Abstract This examined some conditions for the induction of β-D-galactosidase synthesis in Candida kefyr CBS 834. The optimal pH, temperature, and inoculum size either for growth or β-D-galactosidase synthesis were 5.5, 30℃ and above 0.2 at A610nm, respectively. Enzyme activity began to increase at 2h after the addition of inducer, and continued to increase linearly up to 2~3h before reaching stationary phase, and thereafter its activity was decreased. β-D-galactosidase was induced either by lactose or galactose but not either by glucose or ethanol. The greater activity of β-D-galactosidase on galactose than on lactose indicated that the former might be natural inducer for β-D-galactosidase synthesis. The rate of its induction as a function of lactose concentration showed that enzyme activity increased linearly above 4mM, while it was very low below that. Glucose represed the induction of β-D-galactosidase, and the period of adaptation to inducer from other carbon sources was relatively short.
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