| Title | Plamid pEC-3의 중합에 필요한 부위의 동정 | ||
| Author | 장승기 · 이하규 · 노현모 | ||
| Address | 서울대학교 자연과학대학 동물학과 분자유전학 연구실 | ||
| Bibliography | Korean Journal of Microbiology, 22(3),183-190, 1984 | ||
| DOI | |||
| Key Words | Identification of Responsible Region for the Polymerization of Plasmid pEC-3 | ||
| Abstract | In order to find specific acting site of Rec A protein in plasmic polymerization in E. coli, we randomly deleted various part of pEC-3 (a derivative of pBR322) with SI nuclease treatment. Self-ligated plasmids were introduced into E. coli WA802(Rec A+). A number of colonies were analyzed if they contained monomeric or polymeric plasmids by gel electrophoresis. The plasmid (pEC-43), which was deleted the region of tetracycline gene, revealed only monomeric form in Rec A+ E. coli. When two plasmids, pEC-3 and pEC-43, were co-transformed in the same E. coli, the original pEC-3 showed polymerization but pEC-43 revealed monomeric form only. These results suggest that Rec A protein requires the specific site for polymerization. | ||
| Download PDF | Kor_220307_183-190p.pdf | ||