Title 섬유소 분해균인 trichoderma koningii의 분생자 원형질체 융합에 관하여
Author 홍순우 · 하영칠 · 박희문
Address 서울대학교 미생물학과
Bibliography Korean Journal of Microbiology, 22(4),207-214, 1984
Key Words The Conidial Protoplast Fusion of Cellulolytic Fungus, Trichoderma koningii
Abstract Improved methods for the isolation and purification of conidial protoplast were investigated and conidial proplast from auxotrophic mutants were fused. The reaction time for isolation of protoplasts from the swollen condiospores preincubated in liquid minimal medium supplimented with 2-deoxy-D-glucose was shorten by reaction with mixture of 2% driselase and 2% β-glucuronidase(1:1). The conidial protoplast could be highly purified by using 5% Ficoll 400 as a centrifugation medium. Nucleus of the conidial protoplast was stained with Giemsa stain and the conidial protoplast had one nucleus. It was also confirmed that the conidial protoplast was true protoplast with no cell wall remnant at the outside of plasma menbrane. Fusion frequencies of the conidial protoplast from auxotrophic mutants ranged from 3.4×10^-1 to 4.9×10^-1. These values were higher than those of mycelial protoplast by a factor of 5 to 28.
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