Title Klebsiella pneumoniae에 있어서의 Escherichia coli K-12 trpL(Δatt) trpE^FBR 유전자의 클로닝 및 발현
Author 지연태 · 김익영 · 이세영
Address 고려대학교 농과대학 농화학과
Bibliography Korean Journal of Microbiology, 22(4),229-234, 1984
Key Words Cloning and Expression of Escherichia coli K-12 trpL(Δatt) trpE^FBR gene in Klebsiella pneumoniae
Abstract A modified C. coli trp operon, trpL(Δatt)trpE^FBR, was conjugally transfered into Klebsiella pneumoniae KC 100 (Phe^-, Tyr^-, Trp^-, Rif^r, Kam^r) by in vivo cloning using the hybrid plasmid R_6K:: Mucts 61 with a transfer frequency of 5.2×10^-7. Two K. pneumoniae transconjugants, KUA701 and KUA702, were isolated. The characters of attenuation control-free and resistance to feedback-inhibition which are characteristics of donor C. coli trp operon were normally expressed in the KUA 701. However, KUA 702 retained only the feedback-inhibition resistant character. Trp^+ phenotype and ampicillin resistant character were completely stable in the transconjugants, but streptomycin resistant character was lost in the transconjugants.
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