Title Corynebacteria-E. coli shuttle vector pKU6의 분리 및 확인
Author 허태린 · 이진우 · 이세영
Address 고려대학교 농과대학 농화학과
Bibliography Korean Journal of Microbiology, 22(4),249-255, 1984
Key Words Isolation and characterization of Corynebacteria-E. coli shuttle vector pKU6 from Coryneform Bacteria
Abstract To develop the host-vector system for industrial Coryneform bacteria that seemed to be the most suitable microorganisms for molecular breeding of genes involved in the production of amino acids, nucleotides, and other products of industrial interest, broad host range E. coli plasmid R 1162 DNA was transformed into Brevibacterium ammoniagenes and the plasmids pKU6 isolated from a transformant was physically characterized. All other plasmids from the transformed cells except pKU6 exsisted as multimeric forms in Brevibacterium ammoniagenes. The plasmid DNA was retransformed into Corynebacterium glutamicum with a high frequency (1.32×10^-1 per cell) and maintained stably both in Brevibacterium ammoniagenes and Corynebacterium glutamicum after 100 generations of cultures with 25-30 copy number per cell. The size of both plasmid pKU6 and plasmid R1162 were the same and restriction maps by EcoR I, Ava I, Pst I, Pvu II and Hinc II were also similar.
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