Title 세포의 Cytosine Deaminase 생산균 Arthrobacter sp. JH-13의 분리 및 효소생산 조건
Author 전홍기 · 박정혜
Address 부산대학교 자연과학대학 미생물학과
Bibliography Korean Journal of Microbiology, 22(4),257-264, 1984
Key Words Isolation of Extracellular Cytosine Deaminase Producing Strain Arthrobacter sp. JH-13 and Cultural Conditions of it's Enzyme Production
Abstract A strain producing an extracellular cytosine deaminase was isolated from soil samples. The enzyme obtained from the strain possessed the substrate specificity to both cytosine and 5-fluorocytosine. From the results of its morphological, cultural, physiological, and biochemical properties, the strain was thought to be the genus Arthrobacter. Therefore, it was named as Arthrobacter sp. JH-13. The composition of optimum medium for the enzyme formation was 0.5% of peptone, 0.5% of meat extract, 0.5% of soluble starch, and 0.1% of KCl. The optimum pH for the enzyme formation was 8.0. When the microorganism was cultured aerobically in the above medium, enzyme production reached at maximum in 54 hours at 30℃.
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