| Title | E. coli pRDI에서의 DAP-영양요구성 변이주 분리 및 동정 | ||
| Author | 이호자 | ||
| Address | 경희새학교 생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 22(4),265-269, 1984 | ||
| DOI | |||
| Key Words | Isolation and identification of DAP-auxotrophs from E. coli pRDI | ||
| Abstract | For the utilization as donor cells of conjugation, DAP-Auxotrophs were isolated from C. coli cells, carrying plasmid p^RD1 with(a) drug resistance makers from Pseudononas (Km^r, Carb^r, Tc^r) and (b) the nif-gene group from Klebsiela. E. coli p^RD1 cells were treated with nitrosoguanidine for the mutagenesis and cephalexin for the isolation of DAP-Auxotrophs. The nature of auxotrophs was verified by suitable biochemical test and checking with 6-cyanopurine as a color indicator for the presence of nif-gene. | ||
| Download PDF | Kor_220409_265-269p.pdf | ||