Title Rhodopseudomonas sphaeroides K-7에 의한 수소생성에 미치는 생리적 조건에 관한 연구
Author 배무 · 이정국 *
Address 이화여자대학교 자연대학 생물학과; * 한국과학기술원 응용미생물연구실
Bibliography Korean Journal of Microbiology, 21(3),109-114, 1983
DOI
Key Words A study on physiological conditions for hydrogen evolution by rhodopseudomonass phaeroides K-7
Abstract Physiological conditions for hydrogen evolution by Rhodopseudomonas sphaeroides K-7 are examined. Larger amount of molecular hydrogen was evolved at 30℃, pH6.8-7.0 under anaerobically illuminated condition of about 12,000 lux by the organism. The heighest rates of hydrogen evolution were observed in the culture with the organic acids such as acetate, DL-lactate or DL-malate in media containing L-glutamate as a nitrogen source. Hydrogen was also evolved from glucose with the rate of 178.9㎕/hr/㎎ cells (dry weight). When glucose was adopted a sole carbon source, however, considerable time lag of about 20hours was required for hydrogen evolution. The resting cells stored at 0℃ under argon maintained the rate of hydrogen evolution in nearly about 90% of initial one even 40 days of storation.
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