| Abstract |
Effects of temperature on the growth characteristics and the chemical composition of pseudomonas cells grown under glucose-or methanol-utilizing continuous culture were studied. In a glucose-utilizing continuous culture, optimum dilution rate, agitation, pH, and temperature, for the higher biomass yield were 0.45hr, 7000rpm, pH 7.5, and 30℃, respectively. But in a methanol-utilizing continuous culture, they were 0.125hr-, 600rpm, pH 8, and 30℃, respectively. In methanol-utilizing continuous culture, the maximum production rate of the cells was 1.48g, dry wt./1/hr at a dilution rate of 0.45hr-, and the cell yield was 0.46g. dry wt./g. glucose. In the methanol-utilizaing continuous culture, the maximum production rate of the cells was 0.33 7g. dry wt./1/hr. at a dilution rate of 0.125hr- and the cell yield was 0.44g dry cell/g. methanol. The contents of protein of the cells increase with the increase ingrowing temperature (from 15 to 30℃), more or less, while the contents of RNA and carbohydrate of the cells decreased. However, DNA contents of cells growth under the various temperature ranges didn't change. As the temeperature of cultivation rises at a constant dilution rate, the efficiency of RNA in protein synthesis was increased, showing the decreases in the ratio of RNA to protein. |
