| Title | Trichoderma koningii의 conidiospore로부터의 원형질체 분리에 관하여 | ||
| Author | 박희문 · 홍순우 · 하영칠 | ||
| Address | 서울대학교 자연과학대학 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 21(4),213-220, 1983 | ||
| DOI | |||
| Key Words | Isolation of protoplast from conidiospore of Trichoderma koningii | ||
| Abstract | Conditions for isolation of protoplasts from conidiospores of Trichoderma koningii ATCC 26113 were tested. Maximum production of conidial protoplasts was obtained by preincubation of conidiospores on liquid minimal medium for 8 1/2 hrs. and by reaction with cell wall lytic enzyme for 3 hrs. Among effective cell wall lytic enzymes (Driselase, β-Glucuronidase, Novozyme and Zymolyase), Driselase was the most effective one on the production of conidial protoplasts. The production of conidial protoplasts was also enhanced by addition of 2-Deoxy-D-Glucose (25㎍/ml) into liquid minimal medium. Over 70% of the initial swollen conidia, preincubated in liquid minimal medium supplemented with 2-Deoxy-D-Glucose (25㎍/ml), were converted to protoplasts by incubation with 2% (w/v) commercial lytic enzyme Driselase at 28℃ for 3 hrs. The reversion frequency of the conidial protoplasts was about 30 times (25-50%) higher than that of mycelial protoplasts (0.6-1.3%). | ||
| Download PDF | Kor_210405_213-220p0.pdf | ||