| Title | Aspergillus nidulans의 tRNA 유전자의 구성과 발현에 관한 연구 II | ||
| Author | 이병재 · 강현삼 | ||
| Address | 서울대 자연대 미생물학과 | ||
| Bibliography | Korean Journal of Microbiology, 21(4),229-237, 1983 | ||
| DOI | |||
| Key Words | Studies on the Organization and Transcription of Asperfillus nidulans tRNA Genes; II. Cloning of A. nidulans total tRNA genes in Escherichia coli | ||
| Abstract | Total tRNA genes from Aspergillus nidulans were cloned for the further investigation of the structure and expression of Aspergillus tRNA genes. Aspergillus DNA was isolated from spores and cloned into pBR322 plasmid DNA using BamHI and T₄ligase. The recombinant hybrid DNA was transformed into E. coli HB101 and some 30,000 transformants were initially selected. Of these, about 5,300 E. coli clones containing Aspergillus DNA inserted into plasmid pBR322 at BamHl site have been isolated. The hybridization data obtained from the labeled Aspergillus ³²P-tRNA indicated that 105 colonies carried the total tRNA genes. From the data above and cohybridization experiment, tRNA genes of Aspergillus nidulans seem to be twice more clustered than those of yeast. | ||
| Download PDF | Kor_210407_229-237p.pdf | ||